But what is CRISPR-Cas9? An animated introduction to Gene Editing. #some2

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Published 2022-07-12
This CRISPR animation visualizes how the CRISPR/Cas immune system was identified in bacteria and how the CRISPR/Cas9 system was developed for genome editing. This CRISPR animation also covers the history of gene editing, including zinc finger nucleases (ZFN), transcription activator-like effector nuclease (TALE nucleases, TALEN). A history of Jennifer Doudna, Emmanuelle Charpentier, Francisco Mojica, David Liu, and their contributions are described, such as the development of base editing and prime editing. We also go beyond just cutting DNA and discuss how CRISPR turns gene expression on (CRISPR activation, CRISPRa) and off (CRISPR inhibition, CRISPRi) to fluorescently tagging particular sequences (e.g. GFP). This animation explores some of the exciting possibilities of CRISPR-Cas9 biotechnology, including designer babies, genetically modified humans, the end of diseases, and genetic engineering.
But what is CRISPR-Cas9? An animated introduction to Gene Editing.

This video was made by Harrison Ngue, student at Harvard University, as the final project for the course Chemistry 177: The Chemistry, Biology, and Societal Implications of Genome Editing, taught by Professor David Liu and Aditya Raguram. Special thanks to both for providing feedback on this video!

Contents:
0:00 What is Gene Editing?
2:35 Discovery of CRISPR
5:31 CRISPR-Cas9 Technology
7:01 PAM Sequence
8:03 Modern Gene Editing

These biology animations and chemistry animations are largely made using Adobe Premiere Pro and Adobe After Effects, along with the 3blue1brown custom Python library, Manim. Icons, drawing, and chemical structures were designed using BioRender and ChemDraw.

Music:
Pure Water by Meydän
Link:    • Meydän - Pure Water [Creative Commons...  
Forever Sunrise - by Jonny Easton
Link:    • Forever Sunrise - Soft Inspirational ...  
Check out his channel
Link: youtube.com/jonnyeaston

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Powerhouse of the Cell (formerly Primo Science), founded by Harrison Ngue, aims to teach science using animation and a visuals-first approach. If you want to stay posted on new videos, subscribe.

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Twitter: twitter.com/Powerhouse_YT

All Comments (21)
  • @ObamaBinLaden525
    That "maybe that scientist who will accomplish these feats in the future is watching this video right now." was super motivating to me. Thanks!
  • @user-tz7ft5po8w
    I'm a Korean high school student who was searching for terms used in gene editing and I was getting tired. BUT I was so lucky to watch this video and learn much about the overall knowledge of this field. You were just integrating all seperate information I was studying myself. This video is literally "Perfect". Thank you for making and sharing this high quality video. It was so helpful to me
  • This has been so far the most creative science video I've come across. You're literally genius for putting the whole concept in such a perfect sequence and narrating it so gracefully. You literally broke those stereotypes about how science ain't easy to understand. I mean this really is Exceptional !!! Thank you soo much for such amazing efforts 💖
  • @podunkest
    While this has definitely gotten a lot of attention and recognition, including a Nobel prize, I still feel like this is such a monumental discovery their names should be household names up there with Darwin, Einstein and co. It's one of those things that you'd never figure out yourself in a million years but is so elegant and conceptually simple to grasp once it's shown to you.
  • @jarniwoop
    I've been reading about CRISPR's accomplishments for years, and it is nice to read a good description of the process. I did have to watch it twice.
  • @roopesh732
    This is the great explanation of crisper gene editing I've ever seen 👏
  • @JohnSmithXL
    It's always nice to find a science channel that doesn't put me to sleep, keep up the great work!
  • @pikulicluka
    Awesome stuff, I just discovered your channel and I'm stoked to watch all your other videos too. That was a great explanation of how CRISPR-Cas9 works!
  • @Ryan-nh3mt
    I know that I'm late to the party, but WHY IS THIS NOT MORE POPULAR. These videos are ELITE. #1 🎉
  • Dammn that last sentence "maybe that scientist who will accomplish these feats in the future is watching this video right now." felt really emotional for some reason. Thanks
  • @Ryan-nh3mt
    Please keep these coming! They are the best I've seen on here!
  • @hanknew9685
    Utterly fascinating and presented with outstanding clarity and thought. Left me wanting more & more! This channel has some of the most well produced content right now, thank you for the effort and care you put into its creation
  • I love how visual these videos are. This really helps make the abstract relatable
  • This video is absolutely phenomenal and inspiring too. Thanks for explaining the crispr tech crystal clear and with the end note was so motivating. Great job!
  • @davidrosen5137
    Best explanation and animation of CRISPR I've seen. Thank you for putting this together. It's been extremely helpful.
  • What a wonderful way of explaining. Nice animations and on point. Keep up like that and thank you!
  • @llawliet666
    Wow. This is a beautiful video. Incredibly educational, well explained, motivational, and animated. Thanks for making this I guess. My teacher actually recommended this video because we weren't really understanding CRISPR and this helped so much. So, on the off chance you're reading this, thank you. I feel like thank you doesnt cut it but that's what english allows me
  • @OkNowImWatching
    Amazing video! Thank you for taking the time to get everything in order and drawn out clearly
  • @positpixel
    Great video and channel! Love the detailed explanation of how the Cas9 mechanism works.
  • @moinmoin6273
    I wish there were more videos out there that were this simple and nicely explained! Loved this! keep up the great work